Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation.

نویسندگان

  • Sebastian R Sørensen
  • Jim Rasmussen
  • Carsten S Jacobsen
  • Ole S Jacobsen
  • René K Juhler
  • Jens Aamand
چکیده

A bacterial community from Danish agricultural soil was enriched with linuron [N-(3,4-dichlorophenyl)-N'-methoxy-N'-methylurea] as the sole carbon and nitrogen source. The community mineralized [ring-U-14C]linuron completely to 14CO2 and 14C-biomass. Denaturing gradient gel electrophoresis analysis and cultivation revealed that a Variovorax sp. was responsible for the mineralization activity.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 71 7  شماره 

صفحات  -

تاریخ انتشار 2005